Mites are involved in the transmission of many diseases in humans and animals serving as vector. Our research highlights the diversity of mites from 2 tehsils of Pune district.  Three species of phoretic mites, belonging to the order Mesostigmata, were found on beetles (Coleoptera). Abandoned Baya weaver nests collected near the cultivated farms and water bodies showed the presence of three species from order -Mesostigmata, Astigmata and Prostigmata. House dust samples screened from various rural regions were reportedly found to contain a greater number of astigmatid mites, including two different species, and one prostigmatid species. Preference of attachment of Phoretic mites on the host body and morphometric measurements of mite’s body were also recorded.