Agrochemicals are widely used in agriculture to improve crop productivity, but their excessive use leads to soil contamination and affects soil organisms. Earthworms play an important role in maintaining soil fertility and are considered good bio-indicators of soil health. The present study was conducted to assess the toxicity of selected agrochemicals on enzymatic and hematological responses in Pheretima posthuma using vermicompost as a test medium. Adult earthworms were exposed to different concentrations of agrochemicals mixed in vermicompost. Behavioral changes, enzymatic activities, and hematological parameters were evaluated after exposure. The results indicated significant alterations in enzymatic activities and hematological parameters in treated groups compared to control. The findings suggest that agrochemical exposure adversely affects physiological functions of earthworms. Vermicompost medium proved suitable for evaluating soil toxicity. The study emphasizes the need for cautious use of agrochemicals to protect soil fauna and maintain ecological balance.