ARCHIVES
VOL. 10, ISSUE 3 (2025)
Isolation, characterization, and optimization of protease-producing actinomycetes from Rhizosphere soil
Authors
Pratyusha kumar sahu
Abstract
Proteases are industrially significant enzymes with diverse
applications in food processing, pharmaceuticals, leather treatment, and waste
management. In this study, three Actinomycetes isolates were obtained from
rhizosphere soil samples using starch casein agar. Screening on skim milk agar
revealed clear proteolytic zones, indicating extracellular protease production.
Morphological examination confirmed Gram-positive characteristics, and
biochemical profiling demonstrated positive results for methyl red, Voges–Proskauer,
catalase, starch hydrolysis, and multiple carbohydrate fermentations.
Optimization studies revealed that maximum enzyme activity was achieved at 40
°C (137.22 U/L), pH 7.5 (140.20 U/L), and in the presence of CuSO₄ (157.22
U/L). Protein estimation using the Lowry method determined the concentration of
crude enzyme extracts, and SDS–PAGE analysis indicated a molecular weight of
approximately 42 kDa for the purified protease. These findings highlight the
potential of Actinomycetes as a source of industrial proteases and provide
optimized culture conditions to enhance enzyme yield for possible commercial
applications.
Download
Pages:105-110
How to cite this article:
Pratyusha kumar sahu "Isolation, characterization, and optimization of protease-producing actinomycetes from Rhizosphere soil". International Journal of Zoology Studies, Vol 10, Issue 3, 2025, Pages 105-110
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.