The aim of the present investigation was to prepared from two different vermicompost. The experiment was conducted at the Vidya Pratishthan, Baramati campus in biofertilizer unit, during February to April in 2023. Vermicompost was prepared from cow dung and aquatic weeds i.e. Azolla by using Eisenia fetida species of earthworms. Vermiculture was carried out with two different types of sources such as animal excreta and plant biomass. Vermicompost was prepared conducting two different substances i.e. Cow dung and Azolla. These vermicompost were tested for moisture content, pH, nitrogen, carbon, potassium, phosphorus, ash, organic matter, Zinc, Iron, Manganese and Copper. The experimental results show significant variation between Physico-Chemical properties of both vermiculture bed. There was higher Physico - Chemical content observed in Azolla vermicompost. The study also confirmed that, the vermicomposting can produce high quality fertilizer which are better compared to other commercial fertilizer in the market.