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International Journal of
Zoology Studies
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VOL. 8, ISSUE 3 (2023)
Evolutionary relationship between butterfly species of family Nymphalidae using RAPD-PCR
Authors
Pallavi V Singh
Abstract
The present study was undertaken to evaluate genetic diversity and evolutionary relationships among 16 Nymphalidae species belonging to 4 subfamilies viz., Nymphalinae Danainae, Satyrinae and Biblidinae from regions of Amravati. The 9 RAPD markers were screened and the genetic diversity in the form of dendrogram based on binary matrix data set was inferred. Seven of the nine markers generated polymorphic bands, with an average of 22.14 bands per primer scoring 100% polymorphism. The resolution of phylogenetic tree was inferred by Maximum Parsimony and Neighbor-joining methods. The dendrogram exhibits species clustering pattern. Based on binary data matrix the genetic diversity of the species was assessed and it has been observed that numerous taxa exhibit inter-species sister clades that demonstrated shared ancestry. Taxa V. cardui, E. core, and J. lemonias formed outgroup, showing evidence of distant resemblance to other species.
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Pages:89-93
How to cite this article:
Pallavi V Singh "Evolutionary relationship between butterfly species of family Nymphalidae using RAPD-PCR". International Journal of Zoology Studies, Vol 8, Issue 3, 2023, Pages 89-93
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