Tetranychus urticae Koch, the two-spotted spider mite (Acari: Tetranychidae), is a major pest of mites in Egypt and most countries. This pest is currently controlled by synthetic acaricides. However, due to their potential impact on human health, environmental effect, and the potential for undesirable residues, the continued use of synthetic chemicals in different crops, is a growing concern around the world. Therefore, there was a need to improve new biological control methods for the management of mites. Essential oils may represent easy, effective, and natural alternatives as they have a minimum risk. Our study aimed to assess the acaricidal properties of three plant oils (eucalyptus oil, thyme oil, and garlic oil) in vitro. Semi-field tests were also used to determine the sensitivity of T. urticae adult females to various tested oils compared to the recommended pesticide Abamectin 1.8 EC. Mortality was detected after using four concentrations (1%, 0.5%, 0.25%, and 0.125%). Mite mortality was increased by increasing the concentration. In vitro, garlic oil was the most effective compound with a value of LC50 (0.0912 %) followed by Thyme oil, and Eucalyptus oil, with values of LC50 (0.182 and 0.232%), respectively 7 days after treatment. Under semi-field conditions all tested oils reduced mite numbers, nevertheless, Abamectin was found to be the most potent treatment against the spider mite, T. urticae as it caused a total reduction of 68.169% followed by eucalyptus oil where a value of total reduction was 65.741 %. Thyme and garlic oils were less toxic to T. urticae with a total reduction of 55.386 &55.002 %, respectively. Eucalyptus oil, thyme oil, and garlic oil are useful to control the two-spotted spider mite T. urticae. They are hopeful candidates for tetranychids’ biological control. Our study deduces the research directions needed to major knowledge and full evaluation of natural plant oils for future applications in IPM programs.