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VOL. 8, ISSUE 1 (2023)
Genetic characterisation of silurid species based on mtDNA marker- cytochrome B gene
Authors
Mohd Imran
Abstract
The work deals with the divergence and
substitution pattern in the cytochrome b gene and its primary protein structure
in siluriformes species by tracing the variabilities at all three positions of
triplet nucleotide codons of cytochrome b gene (cyt. b) on the basis of the
nucleotide substitutions and their location in the triplet codons of the
resulting amino acid sequence. The maximum Pairwise divergence was observed in
between S. Seenghala and H. fossilis (0.2203) and the minimum was
found between C. gariepinus and H. fossilis (0.1602). The 3rd
nucleotide codon position exhibits the highest substitution rate where the evolutionary
divergence followed as maximum at 3rd nucleotide codon position trailed
by the 1st nucleotide position and the minimum on 2nd nucleotide
codon position. At protein level there were twelve such amino acid locations where
happened the amino acid substitutions as a result of nucleotide substitutions
at triplet codons. Three diverse variabilities were noticed on three different codon
positions of cyt. b gene triplet codons- Nucleotide substitutions: 3rd
(87) >1st (32) >2nd (03); amino acid substitution: 1st
(11) >3rd (04) >2nd (02); and non-conservative
amino acid replacements: 2nd (1:2) >1st (5:11) >3rd
(1:4). Hence, the study has provided an in-depth understanding of the relative
variability and substitution trends in the mitochondrial cytochrome b gene in
catfish species at both DNA and protein level.
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Pages:63-67
How to cite this article:
Mohd Imran "Genetic characterisation of silurid species based on mtDNA marker- cytochrome B gene". International Journal of Zoology Studies, Vol 8, Issue 1, 2023, Pages 63-67
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