Study of effect of Tridax leaf extract on mitochondrial activity of chick embryo hepatic cells
Kishor Dhanji Pendharkar, Anukriti N Nigam
A plant Tridax procumbens (Linn.) is very commonly used as an herbal medicine in healing of wounds. Mitochondria are very important organelle of the cell, by considering the medicinal use of Tridax the purpose of research is to study the effect of leaf extract on mitochondria of the hepatic cell in early chick embryogenesis. The H.H stage 34, i.e., 8days;192hrs incubation was selected because of developed hepatic tissue in the stage 34. (Hamburger Hamilton) . The embryonic characters of this stage ware observed and found that the mitochondrial activity is very important in chick embryo model. Therefore, the effect of extract was studied on the activity of mitochondria. The principle of this assay is succinate dehydrogenase converts succinate to fumarate and transfers the electron to an artificial electron acceptor (Probe), 2,6-dichlorophenolindophenol (DCPIP) (C12H8NO2Cl2), which changes the colour from blue to a colourless product (Jared Rutter et al) . Light transmittance measured using a spectrophotometer. The biochemical study of hepatic SDH activity under control and treated was performed to see the effect of Tridax extract at hepatic mitochondrial level. Our investigation reveals that the controlled sample shows gradual change in absorbance and Tridax extract treated sample showing more change in absorbance and high concentration of extract do not show the change in the absorbance. In this study, we propose a strategy that study of mitochondrial characterization using DCPIP and its oxidation and reduction to characterize aspects of mitochondria has been the feasible approach for analysing properties of Tridax extract as herbal medicine. (Ramesh Petchi et al) .
Kishor Dhanji Pendharkar, Anukriti N Nigam. Study of effect of Tridax leaf extract on mitochondrial activity of chick embryo hepatic cells. International Journal of Zoology Studies, Volume 8, Issue 1, 2023, Pages 6-11