International Journal of Zoology Studies

International Journal of Zoology Studies


International Journal of Zoology Studies
International Journal of Zoology Studies
Vol. 7, Issue 1 (2022)

Alloxan induced changes in gut microflora in Drosophila melanogaster: Protective role of creatine


Manjunath P, Mysore Siddaiah Krishna

Objective: Food consumed by the organism modifies the gut microbe thereby modulating host physiology which in turn affects health and fitness of an organism. Current research was conducted to understand the effects of Alloxan on gut microbial diversity using D.melanogaster as model organism and also to evaluate the effects of creatine on gut microbial diversity. Pyro sequencing of 16S rRNA isolated from the gut of D.melanogaster raised in control, 3% Alloxan, 5% Creatine, 3% Alloxan + Creatine were used for identification of bacterial species. It was noticed that the species of Acetobacter tropicalis, Acetobacter pomorum and Lactobacillus brevis, Lactobacillus fructivorans and Lactobacillus plantarum showed diversity in terms of Alloxan diet treatment. The density of Lactobacillus and Acetobacter species was high in flies fed with alloxan compared to flies fed with control diet. Further flies fed with alloxan along with creatine showed a significant decrease in the density of all the species of Lactobacillus and one species of Acetobacter. Thus these studies clearly suggest Alloxan treatment has an effect on host physiology which in turn affects density of gut microbe in D. melanogaster. Further, creatine feeding along with Alloxan was responsible in bringing back to normal gut microbial condition in D. melanogaster.
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How to cite this article:
Manjunath P, Mysore Siddaiah Krishna. Alloxan induced changes in gut microflora in Drosophila melanogaster: Protective role of creatine. International Journal of Zoology Studies, Volume 7, Issue 1, 2022, Pages 80-86
International Journal of Zoology Studies