Present study aimed at assessing the diversity of fishes from Panchganga River, Ichalkaranji. As Ichalkaranji is well known for cotton industry and due to that the river is polluted, even though during the study total 16 species of freshwater fishes belonging to 4 orders, 10 families and 17 genera were recorded from the sampling sites i.e. Ichalkaranji Bridge, Shiradwad, and Abdul Lat, near Pancha ganga river in Ichalkaranji. Site wise populations and diversity of fish were observed. Among the studied fish species Cyprinidae were found to be 36%, Schilbeidae 11.7% Bagridae 11.7 % and family Ambassidae, Clarridae, Channidae, Gobiidae, Mastacembelidae, Cichlidae and Siluridae showed 5.8 %. Therefore proper scientific management of the aquatic resource for further study regarding the activities affecting the diversity should be carried out.