The present study is carried out to analyze the trace elements sodium, calcium, magnesium, phosphorus, iron, copper, zinc, manganese by atomic absorption spectrometer (AAS) crude protein by Kjeldahl method and essential and non-essential amino acids by reverse phase high performance liquid chromatography (RP-HPLC) from feathers of cock (Gallus domesticus) before the administration of four ACES antioxidant vitamins A, C, E and selenium. There after these four ACES will be mixed with the cock feed and the cock will be kept on blended feed for a period of one month. The feathers of vitamin administered cock (Gallus domesticus) were analyzed for the same mineral, protein and amino acid constituents.