Paraquat effects on gut microbial diversity in Drosophila melanogaster
Krishna M S
In insects gut microbial diversity play important role in host physiology, functions and fitness. Many factors known to affects the gut microbial diversity such as diet, age and environmental factors. In addition to this environmental stressors such as pollutant ie., pesticide and herbicide can also alter the gut microbiota of hosts, which in turn can affect their health and immune systems. Paraquat is one of the chemical pollutant wieldy used in agricultural fields to kill the pest therefore present study has been undertaken to study the effects of paraquaton Gut Microbial Diversity in Drosophila melanogaster. Axon-specific 16S rRNA gene primers for Acetobacter pomorum, Acetobacter tropicalis, Lactobacillus brevis, Lactobacillus fructivorans and Lactobacillus plantarum were used to identify major gut microbial diversity of control and paraquat treated D. melanogaster using primer3 software and unique regions identified from alignments of full 16S rRNA gene sequences. It was noticed that analysis of gut microbial diversity of control and paraquat treated flies revealed two species Acetobacter species group namely Acetobacter pomorum and Acetobacter tropicalis and three species belong to Lactobacillus species namely Lactobacillus brevis, Lactobacillus fructivorans and Lactobacillus plantarum. However their relative abundance varies in control and paraquat treated flies. The density of Lactobacillus species such as L. brevis, L. fructivorans and L. plantarum were found to be lowest in control flies. While these species were highest inparaqut treated flies. Further A. pomorum and A. tropicalis were found to be lowest abundance in control and they were found to be greater abundance inparaquat treated flies. Thus, these studies suggests that significant influence of paraquat treatment on gut microbial density in D. melanogaster.