Physio-chemical parameters of water are important factor for fish health as well as fish production and due to these parameter effect on fish infection by parasite causes different disease to fish. According to Asifa Wali et al., 2016 found that the helminth parasites Pomphorhynchus kashmirensis and Bothriocephalus acheilognathi parasite, 136 (30.63%), 144(32.43%) infect to fishes at different physicochemical parameter that is temp 16.38 C°, DO 6.01 ± 0.40, PH 7.91 ± 0.22 and CO2 4.67 ± 1.12. and also found that the infection fish by30.63 % with two intestinal cestodes parasite Bothriocephalus acheilognathi Yamaguti 1934 at water quality condition. Results revealed that physico-chemical parameter of water showed that the positive correlation with fish parasite and their prevalence, intensity and abundance and it is help for further studies on fish parasite occurrence in upper Dudhana Dam fresh water and effect of physico-chemical parameter.