Metritis is the inflammation of endometrium, underlying glandular tissue and muscular layer. The objective of this study was to determine the susceptibility pattern of bacteria isolated from metritis of domesticated animals. In the present study, a total 53 uterine discharge swabs were collected from domesticated animals (n=16), buffalo (n=14), and goats (n=23). From metritis cases 26 isolates identified on the basis of morphological and cultural characteristics were of E. coli, 8 were of Enterococcus spp and 6 isolates of were Acenetobacterlwoffii. Evaluation of antibiotic sensitivity pattern of bacterial isolates indicated that E. coli was resistant to oxytetracycline (88.46). The Enterococcus faecalis showed resistant to oxytetracycline (75%) and ampicillin (75%). The isolates of Acinetobacter lwoffii showed resistance to oxytetracycline (83.33%) and ampicillin (66.66). This study revealed that E. coli is the most commonly isolated bacteria in metritis. Chloramphenicol was the most effective antibiotic against isolated bacteria.