Aim: Determine the effect immune of bufotoxin 7CH in rats belonging to the Balb-c and Wistar strains Methods: An experimental study was conducted. The bufotoxin was maintained in alcoholic solution and applied to rats in doses of 20 units via intramuscular insulin syringe. Observations were recorded. 10 rats from both strains survived and were administered 9 to 10 times a butotoxin dosage. Results: Administering 9 to 10 dosages generated an acute set of symptoms that culminated with their death, without having a meaningful difference among both strains. Conclusions: The bufotoxin 7CH in both rat strains and intervenes in reducing mortality from effect of the bufotoxin. The death of rat strains by overdose of the bufotoxin could mean the strengthening of the immune system derived from the bufotoxin 7 CH homeopathy.