Variation of temperature plays a pivotal role in sericulture by improving the commercial character. The present investigation inferred that the total protein content in the silk gland has been influenced significantly by varying temperature. The maximum level 9.11 µg/mg of protein was noticed in the silk gland obtained from larvae reared at 26°C while minimum protein content of silk gland 5.56µg/mg was recorded in the silk gland of V^th instar larvae of Bombyx mori at 10°C.