The study of prevalence of Gastrointestinal (GI) parasites in Rhesus Macaque conducted by hotspot area search method and collection of fresh fecal samples from Devghat and Ramnagar, Chitwan that lies in Chitwan-Annapurna landscape. The GI parasites are highly prevalent (80%) in Rhesus Macaque. Among those parasites, helminths (72.2%) were more prevalent than protozoans (27.78%). Altogether nine helminths and seven protozoan species of parasites were identified. The infectious rate was more in summer season (protozoan 60%, helminths 75.5%) than winter (protozoan 46.6%, helminths 68.8%). The study reported Taenia sp. (about 8%) from rhesus monkey in Ramnagar area where the monkeys are mainly depended on the garbage and food produced by the hotels, meat shop and other wastes from local households. These empirical findings are useful for primatologists, veterinarians, ecologists, and also applicable to public health issues where such monkeys and human coexist.