Laboratory studies were carried out to analyze the effects of application of plant growth regulators Pix (mepiquat chloride), salicylic acid and Planofix (naphthaleneacetic acid). Cotton was harvested two times and lint of every picking was analyzed separately. The experiment conditions were on seed index, micronaire, fibre maturity, staple length, fibre strength, ginning out turn and seed germination. Results showed that there was no significant effect of plant growth regulators on all above lint quality characters. There was significant harvesting on staple length, whereas the highest staple length was recorded for second harvesting which was 31.00 mm compared with first which was 29.00 mm. The lowest seed germination was recorded in control plots, while the highest seed germination was recorded in plots receiving plant growth regulators. The highest values of lint quality characters were recorded on first harvest of crop.