In recent years lot of attention is being diverted towards plant extracts as antimicrobial agent. In this context present study is aimed to evaluate antibacterial properties and phytochemicals in Nigella sativa seeds. The antibacterial activity of solvent extracts of Nigella sativa seeds were tested against gram positive bacteria Staphylococcus aureus and gram-negative bacteria Salmonella typhimurium. Design consists of three replicates, positive and negative controls. Agar well diffusion method was used for culturing. Zone of inhibition was measured in millimetres. Phytochemicals screening was carried out using standard protocols. According to the results, against the bacteria S. typhimurium and S. aureus maximum inhibitory zone was exhibited by chloroform and acetone extract respectively and various secondary metabolites were reported. The current study showed the potentiality of N. sativa seeds for controlling bacterial growth and emphasizes its development in the form of a natural antibacterial agent for the control of pathogenic microorganisms.