Surra, a vector borne disease caused by Trypanosoma evansi, is considered as a major enzootic disease mainly for the dromedary camel. Therefore, a cross-sectional study was conducted to determine the prevalence of camel trypanosomosis and assess the distribution and dynamics of the vectors responsible for transmission of the disease in camels (five localities); sheep and goats in Sennar state and equines in White Nile state, from November 2014 to October 2015. Parasitological examination was conducted using Giemsa stained blood smears. The positives were reported in camels only and with prevalence of 2.9% by blood smear. The prevalence and percentages of camels' infection in five geographical locations, seasons and animal breeds, animal sex and age were scoured and discussed. It could be suggested that well-planned research program should be performed including the relation of the surra prevalence to its vector dynamics, host susceptibility, grazing system and vector-control practices in all affected areas.