Study of haematological alterations induced by exposure to diazinon in Channa punctatus (Bloch)
Somali Sood, QJ Shammi
Pesticides are a necessity these days owing to increasing pathogenic attacks on the plants and fresh water. Hence, to prevent such contamination use of pesticides has been promoted. As a result there subtherapeutic levels have very serious impact on the survival rates of the concerned animal. In the present study, the impact of diazinon, a pollutant of land water habitat, was investigated on fresh water fish Channa punctatus (Bloch). The fish (weight =160± 10g, mean length= 25-40cm) were subjected to 0.1 ppm diazinon and observed for mortality. The alive fishes were then exposed to 5 and 50ppm of diazinon concentration. At the end of study, blood samples were collected from the caudal tail vessels with 21 or 23 gauze needles and 1 or 3 cc syringe before ventilator response was noticeably depressed. The studies on different doses of diazinon were performed and alteration in hematological parameters were observed. RBC, hemoglobin concentration, decreased after diazinon exposure while WBC, Mean corpuscular volume (MCV), Mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) showed an increase in the values after treatment with diazinon. Our findings showed that hematological parameters can be used as diagnostic indices to evaluate the health status of Channa punctatus after exposure to diazinon.