A study has been carried out to acoustic & morphology based identification to microchiropteran bat species in district Chitrakoot of Uttar Pradesh with special reference to their distribution. Six roosting sites with the four identified species have been recognised on the basis of ecological condition and microchiropteran abundance. Microchiropteran acoustic was assessed by using Baton Bat Detector. For species identification sounds were recorded of chiropterans of various roosting sites by Baton Bat Detector. Bats 1 emit call is for 0.2 to 100 milliseconds. Body size and activity of larger species increased with altitude, call frequencies increased with the body size of bat species. This is frequency division detector, that means it take 1 second sound and create 10 second gap and then hence 1 sec sound modulates into 10 second, then hence automatically high range frequency gets down to low range frequency and then we are able to hear bat’s ultrasonic sound.