Propolis is a natural resinous mixture produced by honeybee Apis mellifera from substances collected from parts of plants, buds and exudates. Bees gather propolis from different plants, in the temperate climate zone mainly from poplar. Current biological applications of propolis include formulations for cold syndrome (upper respiratory tract infections, common cold, flu-like infections), wound healing creams, eye treatments, Asthama, treatment of burns, acne, dabities, mouthwashes and toothpastes to prevent caries and treat gingivitis and stomatitis. It also possesses the antifungal activities in ocular and vaginal infections. South Indian Propolis possesses antimicrobial, antifungal, antiviral, immunostimulatory, antiinflammatory, anti-cancer and anti-oxidant activity. Worldwide propolis has a tremendous popularity; but in India, studies over propolis are not done extensively. The need of the hour is to explore its possible Pharmaceutical and Biological activities.