Microglia in ageing rat brain: A quantitative study
Sarika Singh Kushwaha
Microglia are the cells of immune surveillance and performed physiological as well as pathophysiological functions in Central Nervous System (CNS). The aim of this study was to investigate age-related changes of microglia in striate cortex. Six age groups of female Wistar rats of 3, 6, 12, 18, 24 and 30 months were used for this purpose. The quantitative changes of microglia in ageing striate cortex were employed by immunohistochemistry with Iba-1 antibody. This study revealed that the number of microglia was increased as age advanced and showed activated phenotypic profile in the senile rat brain. Nevertheless, the area fraction of Iba-1 stained microglia did not show any significant difference between the age groups due to the proliferation and activation of these cells.