Endophytes are microorganisms which present inner parts of plant tissues, without causing any adverse effect. The purpose of this work is to investigate Biochemical potentials of Endophytic Bacteria from medicinal plant (Punica granatum). Endophytic Bacteria isolates are Clostridium sp, Staphylococcus sp are identified by Staining and Biochemical examination. Biochemical studies on the endophytic bacteria growth medium in the presence of Starch, Catalase Casein, Glucose, Maltose, Manitol and Sucrose. The enzyme activity studied on the endophytic fungi it shows the activity of amylase, protease, lipase, cellulase and bacteria showed the amylase and protease activity.