Fish is inseparable part of Indian economy its play vital role in human nutrition as an important source of protein. Asian seabass, Lates calcarifer is one of the ideal species for diversification of Indian brackish water aquaculture sector which as a good domestic market potential species in Andhra Pradesh. In this study biochemical observations cultured and wild Lates calcarifer were comparatively investigated. The biochemical composition such proteins, carbohydrates, lipids, total ash and moisture content were lower in both cage and pond cultured species when compare with wild Lates calcarifer. However, not much significant differences were found in moisture and ash content between wild and culture. It is an economically important food fish in the coastal districts of Andhra Pradesh and the fish is of uniform size and has a body shape amenable for easy portioning with minimum wastage.