Phylogenetic analysis of tasar silkworm, Antheraea mylitta using SSR and ISSR primers
Renuka G and Shamitha G
The diverse strains of the tasar silkworm, Antheraea mylitta were analysed using the simple sequence repeat anchored polymerase chain reaction (SSR and ISSR -anchored PCR). The SSR and ISSR amplification of 7 silkworm strains/eco-races (16 individuals in each, with seven primers, which generated polymorphism) yielded a total of 887 bands, out which 420 were (47.3 %) polymorphic (SSR). A total of 85 bands were produced out of which 46 were polymorphic (54%)for ISSR. Most of the bands were observed within the range 130 to 1250 bp, which is in accordance with the allelic size of the primers taken for studies. A dendrogram was constructed using POPGENE 1.32 version and WINBOOT (XP Windows) for assessing phylogenetic relations. These results suggest that these markers could be effectively utilised for identifying the genetic variability among tasar ecoraces.