The present work was conducted to generate a primary database on ichthyofaunal diversity of Dudhi River, a tributary of River Narmada, Madhya Pradesh, Central India. River consist of a complex mixture of distinctive habitats, which make it among the most productive and valuable ecosystem on earth. The Dudhi River is a tributary of the Narmada basin, which afford a lucrative field of ichthyological importance. River with its tributaries is a unique type of ecosystem which generally covers different types of climatic zones, landscapes and biogeographically regions. River is the natural drainage system of the land mass of the earth which move continuously. During the present investigation, rich ichthyofaunal diversity was observed in the Dudhi River a tributary of River Narmada represented by 19 fish species, 18 genera, 5 families and 4 orders. The Cyprinidae family is dominant group. Fisheries resources are on the decline in India due to over exploitation and inadequate management of her inland waters. For sustainability of these resources, an adequate knowledge of species composition, diversity and relative abundance of her water bodies must be understood and vigorously pursued.