Resveratrol, the naturally occurring polyphenol compound present in plant kingdom was identified to have significant antimicrobial inhibitory activity against human clinical pathogens which causes harmful diseases to human beings by this antimicrobial study. In this in vitro study, eight strains of bacteria and five strains of fungi were used. Among bacteria,the growth of Pseudomonas aerogenosa and Staphylococcus aureus were highly controlled by resveratrol followed by other strains. In antifungal activity, Aspergillus fumicatus and Aspergillus niger shows high zone of inhibition against resveratrol and no activity was recorded with other tested strains of fungi.