Chakrashila Wildlife Sanctuary has rich floral diversity which provides suitable microhabitats for spider fauna. Taxonomic study on spiders in Chakrashila Wildlife Sanctuary is comparatively nil. The present study was carried out to explore spider fauna in Chakrashila Wildlife Sanctuary. Randomly six numbers of 20x20 m quadrats were used at different sites for the study including standard sampling techniques such as visual searching, hand picking and pitfall traps. Collected specimens, as well as photographs were used for identification. A total of 65 spider species belonging to 47 genera under 16 families has been recorded. Amongst the families, the Araneidae was the most abundant (21 species) with orb weavers being the dominant guild type (43%). The present study has enlightened the status and distribution of spider in Chakrashila Wildlife Sanctuary. However, further study needs to be carried out regarding molecular phylogeny, molecular barcoding etc. for the effective conservation of these species.