The vertical distribution of some physicochemical parameters of Ethiope River in Delta State of Nigeria were studied monthly from February 2003 to January 2005 to determine the existence of stratification in a river contrary to lotic water body uniformity concept. Monthly water samples were collected at 1m depth interval from surface to bottom using a Hydro-Bios water sampler along a longitudinally axis from three stations. Pronounced surface to bottom stratification of the studied parameters with rapid changes were evident in the river. Vertical thermal and oxygen differences from surface and bottom varied between 05- 3 0ºC and 1.0 – 4.0 mg/l respectively. The upper waters were thermally stratified with higher temperatures (28 - 30°C) up to about 3.5m while the bottom waters recorded lower temperatures (25 – 27°C). The Tukey Kramer and Duncan multiple comparison tests also revealed significant differences of these parameters in at all depths some studied stations.