Present investigation has been conducted on Papara dam, Satna of Madhya Pradesh with special reference to its zooplankton in relation physico-chemical characteristics. A total of 13 species of protozoa, 03 species of Porifera, 02 species of Coelenterata, 01 species of Platyhelminthes, 17 species of Rotifera, 04 species of Ostracoda, 10 species of Copepoda, 14 species of Cladocera, in all 64 species of zooplankton were identified from Papara Dam in the year 2012-2013. The investigation on physicochemical characteristics at different sites revealed its alkaline nature, suitable for aquaculture practices. Significant site variations have been recorded due to the interference of sewage and agricultural wastes. Among all the zooplankton groups, Rotifera recorded dominance. Maximum diversity of zooplankton population was recorded at macrophytic sites. Later on there was a sudden fall in the month of February and again zooplankton population increased progressively in the following months and reached to the highest value in the month of June.