Vol. 4, Issue 6 (2019)
Extraction of venom from honey bee in district swat, Khyber Pakhtunkhwa, Pakistan
Author(s): Asif Nawaz, Muhammad Asadullah Khan, Rohi Naz, Sidra Zeb
Abstract: This part of work was performed to identify honey bees, extract and analyzed its venomat Agriculture Research Institute Center Swat, Pakistan during the period of June 2015 to August 2015.Honey bees were collected both in dry and wet conditions, preserved in 70% ethanol which was further used for the microscopic identification. Results including microscopic analysis and scientific identification key revealed that the collected specimens were identified as Apis millifera. Honey bee venom is one of the defensive toxin containing a wide range of pharmacologically active compounds. Two methods are reported for collecting the bee venom includingglandular venom (GV) and venom extracted through the use of electrical stimulation (ESV). In the present work latter, ESV was used to extract the venom from honey bees using artificial collector frames containing series of wires, which stimulates bees to sting for releasing the venom from the venom sac in response to electric shocks. Venom were preserved in dark bottles at 4 OCin order to provide proper protection against light and temperature denaturation. The outcome generated from the present work will provide the phylogenetic analysis of the honey bees.