The contents of Total Protein in the fifth instar larvae of silk worm, Bombyx mori (L) fed with mulberry leaves treated with water solution of Eurhodin
Vitthalrao B Khyade
Eurhodin is well known for addition to some growth media for bacterial and cell cultures. The present Attempt is concerned with the analysis of effect of Eurhodin treatment on the levels of protein in the silkworm, Bombyx mori (L). Aqueous solutions of Eurhodin of different strengths (25 ppm; 50 ppm; 75 ppm & 100 ppm) was utilized to treat the leaves of mulberry. 400 ml of aqueous solution of Eurhodin powder was used to treat 100 grams of fresh mulberry leaves for feeding the group of hundred larvae for each time. Treated mulberry leaves were fed to the fifth instar larvae. The feeding the larvae with treated leaves was carried for first four days. Four feedings was the schedule for each day. For each feeding hundred grams of leaves were used. The larvae fed with untreated & water treated leaves were also maintained. Total protein bioassay was carried out on fifth day through the use of silk glands, fat bodies and haemolymph. The total protein content of silk glands of untreated group of larvae was found measured 22.819 units. Eurhodin was found resulted into increase in the salivary gland proteins (total) from 21 to 113 percent. The total protein content of fat bodies of untreated group of larvae was found measured 19.934. Eurhodin treatment was found resulted into increase in the fat body proteins (total) from 34 to 89 percent. The total protein content of haemolymph of untreated group of larvae was found measured 14.564 units. Eurhodin treatment was found resulted into increase in the haemolymph proteins (total) from 22 to 115 percent. Efficient use of Eurhodin may open a new avenue for “Roseus Silk Protein”.