Histological and histochemical study of liver and pancreas of sea snake, Hydrophis cyanocinctus in Minab Coast of Oman Sea
Mojtaba Ghazizadeh, Rahim Abdi, Solmaz Shirali, Abdolali Movahedinia, Narges Amrollahi
In this research 6 adult sea snake, H. cyanocinctus were collected in November from Minab beaches, located in the Sea of Oman. After reviewing and macroscopic examination and confirmation of animal health were euthanized by Chloroform. Then to access of liver and pancreas animals were dissected. Standard procedure of tissue preparation followed and finally sections stained whit Hematoxylin-Eosin, (H&E) and Periodic Acid Schiff's, (PAS) methods, and were observed with a light microscope equipped with a Dino-Lite lens. Microscopic results showed that liver was covered by a very thin capsules. In histological structure of parenchyma was not observed lobular structures and hepatic cords. In some hepatocytes, the nucleus were displaced toward to the periphery. In the sinusoids areas, there were the community of granular brown to dark colors that clearly showed melanomacrophage centers. Bile ducts along with cuboidal structure viewed in portal tracts. In microscopic structure of pancreas was observed that it was composed of two parts, endocrine and exocrine portion. In endocrine portion were observed alpha, beta and delta cells while exocrine portion was includes of communities of compact serous acini.