International Journal of Zoology Studies
International Journal of Zoology Studies
Vol. 4, Issue 1 (2019)

Evaluation of different plant leaf extracts against Myrothecium roridum causing leaf spot of soybean under in vitro conditions


Meghchand Dewangan, Devendra Nishad

Myrothecium leaf spot of soybean caused by Myrothecium roridum. Myrothecium leaf spot of soybean is occurring in almost all the major soybean growing areas of India causing about 30 per cent yield loss. Initial symptoms of the disease appear as small round or oval, brown spots with dark brown margin on leaves in the infected plant. Since it is an economically important disease, management of the disease plays crucial role. Poisoned food technique was employed for the evaluation of different plant leaf extract in the laboratory. The five medicinal plant species viz., Neem, Karanj, Datura, Ashok, and Besharam were used for antifungal activity. Mancozeb was used as check fungicide. All extract of plants leaf were significantly superior in reducing the radial growth over control. The per cent inhibition in growth of M. roridum was ranged from (48.46 to 84.04%) at 10 DAI. The maximum inhibition in growth at 10 DAI was recorded in the extract of Karanj (84.04%). The minimum inhibition by Besharam (48.46%) and Neem (68.65).The per cent growth inhibition of 15 DAI by different plant leaf extract ranged between (43.83 to 81.96%). The maximum radial growth inhibition was recorded in plant extract of Karanj (81.96%) least by Besharam (43.83%) and Neem (63.52 %). Percent growth inhibition indicated that there was maximum inhibition in growth of M. roridum in Karanj, Mancozeb, Ashok, Datura and Neem and least in Besharam.
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