Vol. 3, Issue 5 (2018)
Studies on effect of temperature on fertilization and hatching rate of Pangasianodon hypophthalmus (Sauvage, 1878) eggs
Author(s): CH Satyavathi, P Bujjamma, Srinivas D
Abstract: Pangasius fish (P. hypopthalamus) is an exotic striped catfish, introduced in India during the year 1997. Farming of P. hypopthalamus is an important aquaculture Andhra Pradesh. The present study is carried out to determine the effect of temperature on fertilization and hatching rates of P. hypopthalamus eggs. This experiment was investigated under controlled temperature hatchery. Stripping was done 6 and 9 hours after hormonal injection with crude pituitary gland extract and Ovatide respectively. The water temperature was controlled with an adjustable electric thermostat heater empowered by a generator while other water quality parameters were kept constant in acceptable ranges. The eggs were mixed with the milt using chicken feather and the fertilized eggs were transferred to hatching pool. The temperature for fertilization and hatching maintained in the range of 24-34oC with 2oC intervals. Fertilization and hatchability of the eggs were significantly highest (p < 0.05) at 28ºC and 30ºC compared to other tested temperatures. The results also reveal that the hatching time decrease when the temperature increases, which indicates a positive correlation between incubation temperature and embryonic development rate. Fertilization and hatchability of P. hypopthalamus eggs are best achieved at temperature range of 28 - 30ºC.