Vol. 3, Issue 3 (2018)
Histopathological alterations induced in gills and liver tissue of Clarias gariepinus (Bruchell, 1822) exposed to sublethal doses of copper sulphate
Author(s): Sabiha Shapoo, Mohd Asad Khan, Mohd Waseem, Shabnam Banoo
Abstract: The present study was carried out to determine the histopathological alterations induced in C. gariepinus when exposed to three different sublethal concentrations of copper sulphate for period of 30 days from October to November 2017 at Department of Zoology and Applied Aquaculture, Barkatuallah University, Bhopal.140 fishes having average weight 60 ± 10 g were stocked in plastic tanks with capacity of 200 L water. Fishes were divided into three groups, Group A and B were treated by 1.5mg/L, 2.0 mg/L, 3.0 mg/L while group C served as control. Gills and liver tissues were fixed by bouin’s solution and sectioned in 7 μm and stained with H & E method for microscopic study. Samples of gills and liver tissues were collected at zero day, 15th day, 20th day and 30th and histopahtological changes were observed. Alterations induced were damages like hyperplasia, telangiectasis and edema, necrosis of second filaments, jerky movement, aneurism, hyperaemia and fusion of second filaments in gills; and cell atrophy, necrosis, fatty degeneration, hyperaemia and bile stagnation at different concentrations of copper sulphate as compared to control groups. Liver and Gills tissue damages were increase with the increase of Copper concentration and days. So, copper had adverse toxicity effects on gill and liver tissues in African Catfish at 1.5 mg/L, 2.0mg/L and 3.0mg/L concentrations.