Vol. 3, Issue 1 (2018)
Antibacterial activity of chloroform extract of neem (Azadirachta indica) against pathogenic bacteria
Author(s): Ramadass N, Subramanian N
Abstract: Azadirachta indica known as neem has insecticide and pesticide properties all over the world. In the present study, antimicrobial activity of Azadirachta indica was evaluated against gram negative pathogenic bacteria (Escherichia coli, Salmonella typhi and Vibrio cholerae) and gram positive bacteria (Bacillus subtilis).The Chloroform leaf extracts of Azadirachta indica showed significant antibacterial activity against Gram positive and negative bacteria such as Escherichia Coli, Salmonella enterica, Staphylococcus aureus and Enterococcus faecalis. The in-vitro antibacterial activity was performed by agar well diffusion method. The (25, 50, 75 and 100µg/ml) leaf extract showed maximum inhibition against Enterococcus faecalis (29mm), Salmonella enterica (30mm). Phytochemical tests were performed and showed that the antibacterial activity of plant Azadirachta indica leaves was due to the presence of phytochemical compounds like such as glycosides, alkaloids, tannins, flavonoids, terpenoids, saponins, triterpenoids, reducing sugar, polysaccharides, phytosteroles and phenols. These works identify the specific ingredients responsible for the effect, purify it and standardize same as a drug against bacteria. The neem leaves extracts of Azadirachta indica showed more inhibition zone against Escherichia Coli, Salmonella enterica, Staphylococcus aureus and Enterococcus faecalis.