Vol. 2, Issue 6 (2017)
A study on phytochemical profiling, antibacterial and anticancer of Psidium guajava L. fruit peel extracts of against MCF-7 cell line
Author(s): Arockia Elizabeth Josephine S, Seetharaman S, Indra V, Lakshmi Praba S, Sheela D
Abstract: The aim of this study is to highlight some of the biological activities of methanol extract of Psidium guajava fruit peel. phytochemical screening of four extracts of P. guajava (Aqueous, Methanol, Ethyl acetate and Hexane) were analyzed, among the four extracts only in methanol more activities were noticed. The results of the methanolic extracts showed the presence of phenol, flavonoids, alkaloids and steroid. Where as in other extracts these compounds were not found. Antioxidant activity of P. guajava revealed the highest scavenging activity in methanol fruit peel extract when compare to other extracts using DPPH Assay. The results of antibacterial activity of the tested methanol fruit peel extract against four pathogenic bacterial strains such as Salmonella typhi (10.5±2.8), Escherichia coli (10.8±2.7), Proteus mirabilis (10.5±2.8) and Shigella flexneri (10.8±2.7) (Gram-ve) followed by disc diffusion method. Anticancer activity of (breast cancer MCF-7) of P. guajava methanol fruit peel extract was carried out MCF-7 cell line against normal cell line. At end of 48 hrs of 125µg/ml, 49.20% cell viability was observed by following MTT Assay. Based on the anticancer activity the functional group of the active components present in the P. guajava fruit peel FTIR spectrum was obtained. From this study it is understood that the active compound present in the P. guajava fruit peel extract could be a potent source of natural antioxidants which are of great importance as therapeutic agent in preventing or slowing down the progress of ageing, age associated oxidative stress and related degenerative diseases. Further research is recommended for better characterization of important constituents responsible for antioxidant and anticancer activity.