Identification of secondary metabolic fingerprint by chromatography and spectroscopy tools provides useful information about qualitative, quantitative and the pattern of the composition of there biomolecules. Recently, Fourier Transforms Infrared Spectroscopy (FTIR) reveals to phytochemical profiles containing overlapping signals from a wide array of the compounds.
Allium sativum samples extracted with the help of the solvent ether continuously was subjected to FTIR to determine whether the garlic can we discriminated on the basis of biochemical profiles. This study was carried out to identify the reactive functional groups present in the garlic powder. This preliminary study implied that the garlic (Allium sativum) would be used as protection of new drugs to the benefit of mankind.