Vol. 2, Issue 5 (2017)
Bioinformatics and RNA secondary structure study of metazoans
Author(s): Bibhuti Prasad Barik, Biswajyoti Narayana Sahoo
Abstract: Metazoa are multicellular eukaryotes including all animals with differentiated tissues. ITS rDNA sequences were used to infer for phylogenetic affiliations among metazoan species. Phylogenetic trees were investigated by different methods and one of most parsimonious trees was selected to infer phylogeny. The tree showed more or less similar species clustered together but did not form distinct clades as per their lifestyles. The result indicated that several species appear to be polyphyletic and several unrelated species appear to share the same clade. Further studies are needed to resolve the deep phylogeny of metazoans and to have a better understanding of the early steps of nervous system evolution. The differentiation was further resolved by generating consensus RNA secondary structures of ITS rDNA gene sequences. Consensus RNA secondary structures analysis of different phyla revealed that species belonging to phylum arthropoda possess highest number of A-U, G-C and U-G pairing. The exceptions may be attributed to their adaptations in extreme environmental conditions. Consensus ITS rDNA gene RNA secondary structures showed that the structural features of all forms were quite distinct and different from each other. This observation supports the phylogenetic inference in which similarly named species clustered together based on their lifestyles. The results of the present study revealed that consideration of ITS rDNA gene phylogeny and its consensus RNA secondary structures could be used as possible a phylogenetic marker among diverse species of the animal kingdom for large scale data analysis.
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