Vol. 2, Issue 5 (2017)
Studies of organophosphate pesticide degrading microorganism isolated from Vaigai River, Madurai
Author(s): KP Karuppaiah, Ramesh U
Abstract: Biodegradation progressions which are based on the breakdown of pesticide by Pesticide tolerance microorganism. Vaigai, the major river of Madurai (course of 258 km) originates at Varusa Nadu Hills flowing through the districts of Theni, Dindigul, Madurai, Sivagangai and Ramanadhapuram. Due to the anthropogenic activities and wide usage of different pesticides in agricultural lands (in the form of spray drift, aerial spray), atmospheric fallout, soil erosion and runoff from agricultural areas directly enter the stream of river. Samples were collected from various areas in Tamil Nadu and analyzed for its Monocrotophos SL 36% degradation. Water samples were cultured on minimal media containing 100ppm/L Monocrotophos pesticide concentration. The isolates were identification by using various techniques like Staining, Biochemical Analysis, Antibiotic Sensitivity and pesticide Sensitivity Tests and molecular characterization. Determination of concentration of pesticide on the bacterial growth OD value UV spectroscope was carried out to confirm degradation. Using HPLC technique, Monocrotophos SL 36% was confirmed.