Vol. 2, Issue 5 (2017)
A comparative study of toxicity evaluation and effects of Moringa oleifera Lam. flowers extract on polytene chromosome of vector (Anopheles stephensi L.) and non vector (Anopheles annularis W.) anopheline mosquito fauna
Author(s): Azra Parveen, Archana Gaur, Arti Prasad
Abstract: The natural plants products provide number of associated benefits that bound pesticides environmental impact due to eco friendly, cost-effective and target specific nature and may be useful in preventing resistance developed in mosquito population. Keeping this fact in mind effects of methanolic extracts of Moringa oleifera Lam. flowers on polytene chromosomes of vector (Anopheles stephensi L.) and non vector (Anopheles annularis W.) anopheline mosquitoes were studied and compared both for dose mortality assessment and visible changes. The larval mortality was observed at 24hrs and 48 hrs of time exposure and LC50, LC90 and probit equation were calculated. The LC50 and LC90 values obtained for Moringa oleifera flowers extract against Anopheles annularis were 47.05mg/l, and 95.61.50mg/l respectively at 48 h of exposure time. And Same for Anopheles stephensi were 80.46 mg/l and 130.14 mg/l. Dose mortality test observation showed that Anopheles stephensi mosquitoes are more resistant as compared to Anopheles annularis. It was also reported that due to the effects of flowers extract, chromosome become diffused, super contracted or clumped in greater intensity in Anopheles annularis as compared to Anopheles stephensi.
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