Vol. 2, Issue 5 (2017)
DNA barcoding of endangered fish Dawkinsia tambraparniei for species identification
Author(s): Vijay Velu, Ramesh Uthandakalaipandian
Abstract: Dawkinsia tambraparniei were captured using mosquito nets during period of mid-October of 2015. Species were less found in period of February to May and rich availability at period of August to December. Fishes of length: 6±0.2cm and weight: 4±0.3g were used for study. Hence it was always misjudged as D. arulius further characterization was carried out. The main objective of this study is to determine species identification to avoid false identification. Genetic characterization of D. tambraparniei with mitochondrial Cytochrome c oxidase I (COI) genes revealed that D. tambraparniei has 99% similarity with D. arulius followed by D. filamentosus, P. denisonii, and P. conchonius with 96, 88 and 86% similarity, respectively. Phylogenetic analysis also showed that Dawkinsia tambraparniei is closely related to D. arulius. A discrete Gamma distribution was used to model evolutionary rate differences among these species were done (5 categories (+G, parameter = 0.6335). The obtained COI sequences of D. tambraparniei have been submitted to NCBI database with the reference of GenBank accession number KM978936.1. The present study concludes that the D. tambraparniei obtained from Tamiraparani river shows species variation against D. arulius. It was accurately and rapidly identified by database provided by bold.
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