The study was undertaken to represent the biodiversity and the present conservation status of fishes at Chalan Beel in Bangladesh. Data were collected on the basis of surveying from local farmers, local fishers and fish retailers of local markets from June, 2014 to December, 2016. Fishes from twenty-three families under eight different order were documented. The richest order and family were cypriniformes and cyprinidae which consisting 35 and 32 percent respectively. Twelve common groups were enlisted in study area. The number of threatened species were 18 (27%) among which the number of Vulnerable, Endangered and Critically Endangered were 8 (12%), 8 (12%) and 2 (3%) respectively. Effective steps should be taken to conserve the threatened species of Chalan Beel from the further extinction.