Vol. 1, Issue 3 (2016)
Effectiveness of Nigella sativa (Ranunculaceae) seed extracts on the transaminase activity in certain tissues of Schistocerca gregaria (Orthoptera: Acrididae)
Author(s): Karem Ghoneim, Khalid Hamadah, Atif El-Hela
Abstract: The present study aimed to investigate the disturbing effects ofrnsublethal concentrations (15.0% and 7.5%) of methanol, petroleum ether andrnn-butanol extracts of Nigella sativa seeds on the Glutamic oxaloaceticrntransaminase (GOT) and Glutamic pyruvic transaminase (GPT) activities inrnhaemolymph and fat bodies of last instar nymphs and newly emerged adult femalesrnof the desert locust Schistocerca gregaria which represents a dangerousrnpest for crops and natural pastures over almost 65 countries. In no certainrntrend, a predominant enhancing effect of extracts was unexceptionally exhibitedrnon GOT activity in haemolymph along the last nymphal instar and in haemolymphrnof adults. GOT activity was remarkably disturbed in fat bodies of nymphs sincernit was induced or reduced, depending on the extract. With regard to fat bodiesrnof adults, methanol and n-butanol extracts exerted contradictory actions,rndepending on concentration. Prevalent inducing effect of N. sativa seedrnextracts had been exhibited on GPT activity in haemolymph of nymphs, regardlessrnto the extract. On the contrary, a general reducing effect on GPT activity wasrndetected, with few exceptions, in haemolymph of adults. Concerning the fatrnbodies of nymphs, GPT activity was enhanced in early-aged nymphs but inhibitedrnin mid-aged congeners, irrespective of the extract. In late-aged ones,rnpetroleum ether and n-butanol extracts exerted reducing actions on the enzymernactivity. In fat bodies of adults, methanol extract exhibited an extendedrninducing effect on GPT activity but other extracts exhibited reducing effectsrnof it.